Amino functional group free#

In test tube C dissolve naphthol in dilute sodium hydroxide.

In test tube B prepare aqueous solution of sodium nitrite solution.

In test tube A dissolve the given organic compound in 2ml of dilute hydrochloric acid.

Simultaneously take three test tubes and mark it as A, B and C.

These diazo compounds couple with phenols like β-naphthols forms an orange azo dye which is insoluble in water. Aromatic amines forms a diazonium salts when treated with HNO 2.

This test is performed in order to confirm the presence of aromatic amines. Note: Appearance of bubbles shows the presence of primary amines, yellow oily liquid shows the presence of secondary amines and formation of nitrite salts conforms the presence of tertiary amines.

Add excess of nitrous acid solution if there is an absence of nitrous acid.

Amino functional group free#

Test the solution for the presence of free nitrous acid.To the cooled solution add sodium nitrite dropwise and mix well.Cool the solution in ice bath for 0-5 oC.Dissolvethe given organic compound in concentrated hydrochloric acid and water in a clean test tube.An aromatic primary amine reacts with nitrous acid forms a diazonium salt and at higher temperatures the formed diazonium compounds undergoes decomposition. This test can be used to identify primary, secondary and tertiary amines. HCl after the test and then throw into sink. Carpytomine (isocyanide) is highly poisonous so do not inhale its vapours.Ģ. Note: An unpleasant odor confirms the presence of primary amine.ġ. Observe the odor of the resulting mixture.To the above mixture add a small amount of given organic compound to be tested and warm gently.To this add a few drops of chloroform and shake well.Warm the test tube until the potassium hydroxide dissolves completely in it.Take potassium hydroxide solid in a clean test tube and add 2ml of ethanol to it.Secondary and tertiary amines do not give this test. Both aliphatic and aromatic amines gives positive for this test. This test is also called isocyanide test, because when amines are treated with chloroform in the presence of alkali, isocyanide is formed.

Note: The change in the colour of red litmus paper shows the given organic compound is a base. Observe the change in the colour of red litmus paper.Place one to two drops of given organic compound on it.Litmus TestĪmines have appreciable basicity and turn red litmus blue.Amines are basic in nature.This test is also not a confirmation test for amines. Note: If it is soluble in mineral acid then it may be an amine. Shake well the solution and observe the solubility.Add 2 to 3 drops of concentrated hydrochloric acid.Take the given organic compound to be tested in a clean test tube.There are 5 tests that can be carried out for amino functional group:Īmines are the organic compounds that have appreciable basicity and so they dissolve in mineral acids like hydrochloric acid.